This example sets up a NF(xz)-shear for an eight element cube with two elements each direction to solve a material parameter estimation process with a forward solution procedure. It only validates the optmisation process. The material is assumed to be orthotropic and modelled by the Criscione material law through the CellMl environment, see also examples 5g* for further information about the implementation of an arbitrary constitutive law. The forward solution procedure is run to obtain synthetic force data of the top face of the cube which is later used for the objective function calculation, i.e. read in as the ipdata file. The optimiser then starts with different starting values and builds the gradient by calling the command file: "222_NF_Optimisation_Loop.com" which simulates the simple shear. As expected the optimiser converges to the values of theta51=0.8949 and theta53=0.2346 as stated in the ipcell file.